Porphyrin cross‐linked conjugated polymer nanoparticles‐based photosensitizer for antimicrobial and anticancer photodynamic therapies

We report here the synthesis and characterization of a water dispersible conjugated polymer nanoparticle-based photosensitizer its application in antibacterial anticancer phototherapies. Nanoparticles (CPPN) were synthesized one-pot by nanoprecipitation method, which hydrophobic azide functionalized, red-emitting thiophene-benzothiodiazole based (CP-AZ) was cross-linked with hydrophilic, propargylamine functionalized porphyrin (TPP-4AL) through cucurbit[6]uril (CB6) catalyzed azide-alkyne cycloaddition (CB6-AAC) reaction. CPPN demonstrated high stability aqueous medium for more than month without any visible aggregation appeared to be good light-triggered reactive oxygen species (ROS) generation ability. Consequently, displayed photo-induced biocidal activity against Gram-negative (Escherichia coli, E. coli) Gram-positive (Bacillus subtilis, B. subtilis Staphylococcus aureus, S. aureus) bacteria. When bacteria suspension incubated (20 μg ml−1) irradiated white light (22 mW cm−2) 10 min, 3.5-log reduction colony-forming units (CFUs) recorded three model minimal dark cytotoxicity Moreover, on mammalian cell studied using MCF-7 breast cancer line. The results that is non-toxic cells even at concentration 112.5 ml−1 this feature makes an ideal photosensitizer.